Below is a list of recent publications from MU that incorporate data generated by the Charles W Gehrke Proteomics Center or utilize Center instrumentation.



NaTrxh Is an Essential Protein for Pollen Rejection in Nicotiana by Increasing S-RNase Activity
Maria D Torres-Rodríguez, Yuridia Cruz-Zamora, Javier A Juárez-Díaz, Brian Mooney, Bruce A McClure, Felipe Cruz-García
Plant J. May 11. doi: 10.1111/tpj.14802

The complex response of free and bound amino acids to water stress during the seed setting stage in Arabidopsis.
Abou Yobi, Clement Bagaza, Albert Batushansky, Vivek Shrestha, Marianne L. Emery, Samuel Holden, Sarah Turner‐Hissong, Nathan D. Miller, Thomas P. Mawhinney, Ruthie Angelovici
Plant J, 102, 4, 838-855

Quantitative proteomic analysis of chikungunya virus-infected Aedes aegypti reveals proteome modulations indicative of persistent infection.
Y Cui, P Liu, BP Mooney, AWE Franz
Journal of Proteome Research 19, 6, 2443-2456

Quantitative proteomics reveals docosahexaenoic acid-mediated neuroprotective effects in lipopolysaccharide-stimulated microglial cells.
Bo Yang, Runting Li, Pei N Liu, Xue Geng, Brian P Mooney, Chen Chen, Jianlin Cheng, Kevin L Fritsche, David Q Beversdorf, James C Lee, Grace Y Sun, C Michael Greenlief
J. Proteome Res 19, 6, 2236–2246

Suppression of Organismal Death Pathway in the Heart Tissues of Diabetic Mice by NP6A4, A Peptide Agonist of the Angiotensin II Receptor AT2R.
L Pulakat, A Belenchia, B Mooney, VG DeMarco
The FASEB Journal 34 (S1), 1-1

Identification of health-promoting bioactive phenolics in black walnut using cloud-based metabolomics platform.
Danh C Vu, Jihyun Park, Khanh-Van Ho, Lloyd W Sumner, Zhentian Lei, C Michael Greenlief, Brian Mooney, Mark V Coggeshall, Chung-Ho Lin
Journal of Food Measurement and Characterization 14 (2), 770-777

Characterization and regulation of extracellular vesicles in the lumen of the ovine uterus
Eleanore V O’Neil, Gregory W Burns, Christina R Ferreira, Thomas E Spencer
Biology of Reproduction, Volume 102, Issue 5, May 2020, Pages 1020–1032

Analysis of the uterine lumen in fertility-classified heifers: II. Proteins and metabolites
Joao G N Moraes, Susanta K Behura, Jeanette V Bishop, Thomas R Hansen, Thomas W Geary, Thomas E Spencer
Biology of Reproduction, Volume 102, Issue 3, March 2020, Pages 571–587

EPSIN1 Modulates the Plasma Membrane Abundance of FLAGELLIN SENSING2 for Effective Immune Responses
Carina A. Collins, Erica D. LaMontagne, Jeffrey C. Anderson, Gayani Ekanayake, Alexander S. Clarke, Lauren N. Bond, Daniel J. Salamango, Peter V. Cornish, Scott C. Peck, Antje Heese
Plant Physiol. 2020 Apr; 182(4): 1762–1775.

Effects of Docosahexaenoic Acid and its Peroxidation Product on Amyloid-β Peptide-Stimulated Microglia
Xue Geng, Bo Yang, Runting Li, Tao Teng, Mary Jo Ladu, Grace Y. Sun, C. Michael Greenlief, and James C. Lee, 
Molecular Neurobiology, 57, 1085-1098 (2020),

Bioactive components from garlic on brain resiliency against neuroinflammation and neurodegeneration (Review)
Hailong Song, Jiankun Cui, Valeri V. Mossine, C. Michael Greenlief, Kevin Fritsche, Grace Y. Sun, and Zezong Gu,
Experimental and Therapeutic Medicine, 19, 1554-1559 (2020),

Harpagophytum procumbens extract ameliorates allodynia and modulates oxidative and antioxidant stress pathways in a rat model of spinal cord injury
Garrett Ungerer, Jiankun Cui, Tina Ndam, Mikeala Bekemeier, Hailong Song, Runting Li, Heather R. Siedhoff, Bo Yang, Michael K. Appenteng, C. Michael Greenlief, Dennis K. Miller, Grace Y. Sun, William R. Folk, and Zezong Gu
NeuroMolecular Medicine, 22, 278-292 (2020),


12-Hydroxy-Jasmonoyl-l-Isoleucine Is an Active Jasmonate That Signals through CORONATINE INSENSITIVE 1 and Contributes to the Wound Response in Arabidopsis
Arati N Poudel, Rebekah E Holtsclaw, Athen Kimberlin, Sidharth Sen, Shuai Zeng, Trupti Joshi, Zhentian Lei, Lloyd W Sumner, Kamlendra Singh, Hideyuki Matsuura, Abraham J Koo
Plant and Cell Physiology, 60 (10) 2152–2166

Adaptive responses of amino acid metabolism to the combination of desiccation and low nitrogen availability in Sporobolus stapfianus
Abou Yobi, Albert Batushansky, Melvin J. Oliver & Ruthie Angelovici
Planta volume 249, pages1535–1549(2019)

Compartmentalization of the Proteasome-Interacting Proteins During Sperm Capacitation
Michal Zigo, Pavla Manaskova-Postlerova, Vera Jonakova, Karl Kerns, Peter Sutovsky
Sci Rep. 2019 Aug 29;9(1):12583.

Comparative Proteomic Analysis Unveils Critical Pathways Underlying the Role of Nitrogen Fertilizer Treatment in American Elderberry
Bo Yang, Andrew L. Thomas, and C. Michael Greenlief,
Proteomes. 7(1): 10.

Maternal Dietary Docosahexaenoic Acid Alters Lipid Peroxidation Products and (n-3)/(n-6) Fatty Acid Balance in Offspring Mice
Bo Yang, Runting Li, Taeseon Woo, Jimmy D. Browning, Jr., Hailong Song, Zezong Gu, Jiankun Cui, James C. Lee, Kevin L. Fritsche, David Q. Beversdorf, Grace Y. Sun, and C. Michael Greenlief
Metabolites. 2019 Mar; 9(3): 40.

Quercetin Potentiates Docosahexaenoic Acid to Suppress Lipopolysaccharide-induced Oxidative/Inflammatory Responses, Alter Lipid Peroxidation Products, and Enhance the Adaptive Stress Pathways in BV-2 Microglial Cells
Grace Y. Sun, Runting Li, Bo Yang, Kevin L. Fritsche, David Q. Beversdorf, Dennis B. Lubahn, Xue Geng, James C. Lee, and C. Michael Greenlief
Int J Mol Sci. 2019 Feb; 20(4): 932.

Tasselseed5 overexpresses a wound-inducible enzyme, ZmCYP94B1, that affects jasmonate catabolism, sex determination, and plant architecture in maize
China Lunde, Athen Kimberlin, Samuel Leiboff, Abraham J. Koo, and Sarah Hake
Commun Biol. 2019; 2: 114.

From Analysis of Ischemic Mouse Brain Proteome to Identification of Human Serum Clusterin as a Potential Biomarker for Severity of Acute Ischemic Stroke
Hailong Song, Hui Zhou, Zhe Qu, Jie Hou, Weilong Chen, Weiwu Cai, Qiong Cheng, Dennis Y Chuang, Shanyan Chen, Shuwei Li, Jilong Li, Jianlin Cheng, C. Michael Greenlief, Yuan Lu, Agnes Simonyi, Grace Y Sun, Chenghan Wu, Jiankun Cui, and Zezong Gu
Translational Stroke Research, 2019; 10: 546-556


Defects in longchain 3hydroxy acylCoA dehydrogenase lead to hepatocellular carcinoma: A novel etiology of hepatocellular carcinoma
Tripti Khare, Sharad Khare, Jerry J. Angdisen, Qiong Zhang, Alexei Stuckel, Brian P. Mooney, Suzanne E. Ridenhour, Raad S. Gitan, Ghassan M. Hammoud, Jamal A. Ibdah
Int J Cancer Mar 1 doi: 10.1002/ijc.32943

A High‐Throughput Absolute‐Level Quantification of Protein‐Bound Amino Acids in Seeds
Abou Yobi and Ruthie Angelovici
Current Protocols, 3 (4), e20084

Docosahexaenoic acid (DHA): an essential nutrient and a nutraceutical for brain health and diseases
Grace Y. Sun, Agnes Simonyi, Kevin L. Fritsche, Dennis Y. Chuang, Mark Hannink, Zezong Gu, C. Michael Greenlief, Jeffrey Yao, James C. Lee, and David Q. Beversdorf
Prostaglandins, Leukotrienes, and Essential Fatty Acids, 136, 3-13 (2018),

Unveiling anti-oxidative and anti-inflammatory effects of 4-hydroxyhexenal and 4-hydroxynonenal and their regulation by docosahexaenoic acid and lipopolysaccharide in microglial cells
Bo Yang, Runting Li, C. Michael Greenlief, Agnes Simonyi, Kevin L. Fritsche, Zezong Gu, Jiankun Cui, David Q. Beversdorf, and Grace Y. Sun
Journal of Neuroinflammation, 15, 202 (2018),


Cell surface Thomsen-Friedenreich proteome profiling of metastatic prostate cancer cells reveals potential link with cancer stem cell-like phenotype.
Feng Li, Olga V. Glinskii, Brian P. Mooney, Kate Rittenhouse-Olson, Kenneth J. Pienta and Vladislav V. Glinsky
Oncotarget, 2017, Vol. 8, (No. 58), pp: 98598-98608

Controlled modification of biomolecules by ultrashort laser pulses in polar liquids.
Gruzdev V, Korkin D, Mooney BP, Havelund JF, Moller IM and Thelen JJ.
2017 Scientific Reports 7, Article number: 5550

Proteomic identification of a potential sex biomarker for 2 fruit fly species at pupal stage.
Chiou Ling Chang.
Journal of Asia-Pacific Entomology, Volume 20, Issue 1, March 2017, Pages 125-131

Data on the phosphorylation state of the catalytic serine of enzymes in the α-D-phosphohexomutase superfamily.
Lee Y, Furdui C, Beamer LJ.
Data Brief. 2016 Dec 15;10:398-405. doi: 10.1016/j.dib.2016.12.017. eCollection 2017.

Discriminant Analyses of the Polyphenol Content of American Elderberry Juice from Multiple Environments Provide Genotype Fingerprint
Mitch C. Johnson, Matheus Dela Libera Tres, Andrew L. Thomas, George E. Rottinghaus, and C. Michael Greenlief
Journal of Agricultural and Food Chemistry, 65, 4044–4050 (2017)


Nucleobase modification by an RNA enzyme.
Poudyal RR, Nguyen PD, Lokugamage MP, Callaway MK, Gavette JV, Krishnamurthy R, Burke DH.
Nucleic Acids Res. 2016 Dec 6. pii: gkw1199. [Epub ahead of print]

Plasma membrane proteomics in the maize primary root growth zone: novel insights into root growth adaptation to water stress.
Voothuluru P, Anderson JC, Sharp RE, Peck SC
Plant Cell Environ. 2016 Sep;39(9):2043-54. doi: 10.1111/pce.12778. Epub 2016 Jul 25

Quantitative Proteomics of Zea mays Hybrids Exhibiting Different Levels of Heterosis.
Dahal D, Newton KJ, Mooney BP.
J Proteome Res. 2016 Aug 5;15(8):2445-54. doi: 10.1021/acs.jproteome.5b01120

Induced Structural Disorder as a Molecular Mechanism for Enzyme Dysfunction in Phosphoglucomutase 1 Deficiency.
Stiers KM, Kain BN, Graham AC, Beamer LJ
J Mol Biol. 2016 Apr 24;428(8):1493-505. doi: 10.1016/j.jmb.2016.02.032. Epub 2016 Mar 10

Method development and validation for quantitation of FruArg in mice plasma and brain tissue using UPLC-MS/MS
Mitch C. Johnson, Hailong Song, Jiankun Cui, Valeri V. Mossine, Zezong Gu, and C. Michael Greenlief
ACS Omega, 1, 663-668 (2016)

Effects of aged garlic extract and FruArg on gene expression and signaling pathways in lipopolysaccharide-activated microglial cells
Hailong Song, Yuan Lu, Zhe Qu, Valeri V. Mossine, Jie Hou, Jiankun Cui, Brenda Peculis, Thomas P. Mawhinney, Jianlin Cheng, C. Michael Greenlief, Kevin Fritsche, Frank J. Schmidt, Ronald B. Walter, Dennis B. Lubahn, Grace Y. Sun, and Zezong Gu
Scientific Reports, 6, 35323 (2016)

Quantitative proteomic approaches for analysis of protein S-nitrosylation
Zhe Qu, C Michael Greenlief, and Zezong Gu
Journal of Proteome Research, 15, 1-14 (2016)


Impact of Frozen Storage on the Anthocyanin and Polyphenol Content of American Elderberry Fruit Juice
Mitch C. Johnson, Andrew L. Thomas, and C. Michael Greenlief
Journal of Agricultural and Food Chemistry, 63, 5653-5659 (2015)

Inhibition of Microglial Activation by Elderberry Extracts and Its Phenolic Components
Agnes Simonyi, Zihong Chen, Jinghua Jiang, Yijia Zong, Dennis Y. Chuang, Zezong Gu, Chi-Hua Lu, Kevin L. Fritsche, C. Michael Greenlief, Andrew L. Thomas, Dennis B. Lubahn and Grace Y. Sun
Life Sciences, 128, 30-38 (2015)

Determination of Anthocyanins and Total Polyphenols in a Variety of Elderberry Juices By UPLC/MS and Other Methods
Hanzhi Wu, Mitch C. Johnson, Chi-Hua Lu, Kevin L. Fritsche, Andrew L. Thomas, Zongwei Cai and C. Michael GreenliefActa
Horticulturae, 1061, 43-51 (2015)

Peptidomics study in anthocyanin abundant juice of elderberry
Hanzhi Wu, Mitch C. Johnson, Chi-Hua Lu, Kevin L. Fritsche, Andrew L. Thomas, Zongwei Cai and C. Michael Greenlief
Talanta, 131, 640-644 (2015)

Role of aA-crystallin-derived aA66-80 peptide in guinea pig lens crystallin aggregation and insolubilization.
Raju M, Mooney BP, Thakkar KM, Giblin FJ, Schey KL, Sharma KK.
Exp. Eye Res. 132:151-60

The Basis of Asymmetry in the SecA:SecB Complex
Yuying Suo, Simon J.S. Hardy, Linda L. Randall
J Mol Biol (2015)

Diethylaminobenzaldehyde Is a Covalent, Irreversible Inactivator of ALDH7A1
Min Luo, Kent S. Gates, Michael T. Henzl, and John J. Tanner
ACS Chem (Jan 2015)


Proteomic analysis of the effects of aged garlic extract and its FruArg component on lipopolysaccharide-induced neuroinflammatory response in microglial cells
Hui Zhou, Zhe Qu, Valeri V. Mossine, Dineo L. Nknolise, Jilong Li, Zhenzhou Chen, Jianlin Cheng, C. Michael Greenlief, Thomas P. Mawhinney, Paula N. Brown, Kevin L. Fritsche, Mark Hannink, Dennis B. Lubahn, Grace Y. Sun, Zezong Gu
PLoS ONE, 9 (11): e113531 (2014), doi: 10.1371/journal.pone.0113531

Proteomic Quantification And Site-Mapping of S-Nitrosylated Proteins Using Isobaric iodoTMT Reagents
Zhe Qu, Fanjun Meng, Ryan Bomgarden, Rosa Viner, Jilong Li, John Rogers, Jianlin Cheng, C. Michael Greenlief, Jiankun Cui, Dennis Lubahn, Grace Sun, and Zezong Gu
Journal of Proteome Research, 13, 3200-3211 (2014), doi: 10.1021/pr401179v

Interstrand DNA-DNA Cross-Link Formation Between Adenine Residues and Abasic Sites in Duplex DNA 
Nathan E. Price, Kevin M. Johnson, Jin Wang, Mostafa I. Fekry, Yinsheng Wang, and Kent S. Gates
J.Am.Chem.Soc., 136, 3483-3490

Covalent Adduct Formation between the Antihypertensive Drug Hydralazine and Abasic Sites in Double- and Single-Stranded DNA
Douglas Melton, Calvin D. Lewis, Nathan E. Price, and Kent S. Gates
Chem. Res. Toxicol., 27, 2113-2118

Atrazine Affects Phosphoprotein and Protein Expression in MCF-10A Human Breast Epithelial Cells
Peixin Huang, John Yang, and Qisheng Song
Int. J. Mol. Sci., 15, 17806-17826

Dietary lufenuron reduces egg hatch and influences protein expression in the fruit fly Bactrocera latifrons (Hendel)
Chiou Ling Chang, Scott Geib, Il Kyu Cho, Qing X. Li, David Stanley

Compromised catalysis and potential folding defects in in vitro studies of missense mutants associated with hereditary phosphoglucomutase 1 deficiency
Yingying Lee, Kyle M. Stiers, Bailee N. Kain and Lesa J. Beamer
J. Biol. Chem. Published online Oct 6th 2014

NitroDIGE analysis reveals inhibition of protein S-nitrosylation by epigallocatechin gallates in lipopolysaccharide-stimulated microglial cells
Zhe Qu, Fanjun Meng, Hui Zhou, Jilong Li, Quanhui Wang, Fan Wei, Jianlin Cheng, C Michael Greenlief, Dennis B Lubahn, Grace Y Sun, Siqi Liu and Zezong Gu
Journal of Neuroinflammation, 11:17

Promotion of Enzyme Flexibility by Dephosphorylation and Coupling to the Catalytic Mechanism of a Phosphohexomutase
Yingying Lee, Maria T. Villar, Antonio Artigues, and Lesa J. Beamer
J. Biol. Chem. DOI:10.1074/jbc.M113.532226


Membrane protein complex of APS reductase and Qmo is present in Desulfovibrio vulgaris and Desulfovibrio alaskensis.
LR Krumholz, L Wang, DAC Beck, T Wang, M Hackett, B Mooney, TR Juba, MJ McInerney, B Meyer, JD Wall, D Stahl (2013) Microbiology 159 (Pt 10), 2162-2168

Proteomic analysis of the testa from developing soybean seeds.
Miernyk JA, Johnston ML. J
Proteomics. 2013 89:265-72

Identification of Subunit-Subunit Interaction Sites in aA-WT Crystallin and Mutant aA-G98R Crystallin Using Isotope-Labeled Cross-Linker and Mass Spectrometry
Rama Kannan, Puttur Santhoshkumar, Brian P. Mooney, and K. Krishna Sharma
PLOS ONE DOI:10.1371

The aA66-80 Peptide Interacts with Soluble a-Crystallin and Induces Its Aggregation and Precipitation: A Contribution to Age-Related Cataract Formation.
Kannan R, Santhoshkumar P, Mooney BP, Sharma KK.
Biochemistry. 2013 May 16. [Epub ahead of print]

Profiling of lens protease involved in generation of aA-66-80 crystallin peptide using an internally quenched protease substrate.
Hariharapura R, Santhoshkumar P, Krishna Sharma K.
Exp Eye Res. 2013 Apr;109:51-9.

Identification of an essential active-site residue in the a-D-phosphohexomutase enzyme superfamily.
Lee Y, Mehra-Chaudhary R, Furdui C and Beamer LJ
The FEBS Journal (online April 8th 2013)

On the formation and properties of interstrand DNA-DNA cross-Links forged by reaction of an abasic site with the opposing guanine residue of 5'-CAp sequences in duplex DNA.
Johnson KM, Price NE, Wang J, Fekry MI, Dutta S, Seiner DR, Wang Y, and Gates KS.
J. Am. Chem. Soc., 135, 1015-1025


aA-Crystallin-derived mini-chaperone modulates stability and function of cataract causing aAG98R-crystallin.
Raju M, Santhoshkumar P, Sharma KK.
PLoS One. 2012;7(9):e44077.

Specific changes in total and mitochondrial proteomes are associated with higher levels of heterosis in maize hybrids
Diwakar Dahal, Brian P. Mooney, Kathleen J. Newton
The Plant Journal, 72: 70-83

A Proteomic analysis of liver after ethanol binge in chronically ethanol treated rats
Annayya R Aroor, Roy J Lowery, Ricardo J Restrepo, Brian P Mooney and Shivendra D Shukla
Proteome Science, 10: 29

Prostaglandin A2 Influences Gene Expression In an Established Insect Cell Line (BCIRL-HzAM1) Cells
Stanley, D.W., Goodman, C., An, S., Song, Q.
Journal of Insect Physiology, 58:, 837-849

Larval Dietary Wheat Germ Oil Influences Age-specific Protein Expression In Adults of the Oriental Fruit Fly
Chang, C.L., Coudron, T.A., Goodman, C.L., Stanley, D.W.
Journal of Insect Physiology, 58: 690-698

Solution NMR of a 463-residue Phosphohexomutase: Domain 4 Mobility, Substates, and Phosphoryl Transfer Defect
Sarma, A.V.S., Anbanandam, A., Kelm, A, Mehra-Chaudhary, R., Wei, Y, Qin, P., Lee, Y, Berjanskii, M.V., Mick, J.A., Beamer, L.J., and Van Doren, S.R.
Biochemistry, 51: 807-019


Mapping the lipoylation site of Arabidopsis thaliana plastidial dihydrolipoamide S-acetyltransferase using mass spectrometry and site-directed mutagenesis.
Jill Casteel, Ján A. Miernyk, Jay J. Thelen
Plant Physiology and Biochemistry xxx (epub ahead of print)

Core unit of chemotaxis signaling complexes
Mingshan Li and Gerald L Hazelbauer
PNAS Vol. 108: 9390-9395

Asp295 Stabilizes the Active-Site Loop Structure of Pyruvate Dehydrogenase, Facilitating Phosphorylation of Ser292 by Pyruvate Dehydrogenase-Kinase.
Tripty A. Hirani, Alejandro Tovar-Méndez, Ján A. Miernyk, and Douglas D. Randall
Enzyme Research Volume 2011, Article ID 939068

Simplified enrichment of plasma membrane proteins for proteomic analyses in Arabidopsis thaliana.*
Zhang ZJ, Peck SC.
Proteomics. 11(9):1780-8

alphaA-crystallin peptide 6680 accumulating in aging lens inpairs the function of alpha-crystallin and induces lens protein aggregation
Puttur Santhoshkumar, Murugesan Raju, K. Krishna Sharma
PLoS ONE 6(4): e19291

Identification and characterization of a copper-binding site in alphaA-crystallin.
Murugesan Raju, Puttur Santhoshkumar, T. Michael Henzl, K. Krishna Sharma
Free Radical Biology & Medicine 50:1429–1436

Phenotypic and gene expression analyses of a ploidy series of maize inbred Oh43.
Hong Yao, Akio Kato, Brian Mooney and James A. Birchler
Plant Molecular Biology. 75:237-251 (published on-line December 2010)


NMR and Bioinformatics Discovery of Exosites that Tune Metalloelastase Specificity for Solubilized Elastin and Collagen Triple Helices
Mark O. Palmier, Yan G. Fulcher, Rajagopalan Bhaskaran, Vinh Q. Duong, Gregg B. Fields, and Steven R. Van Doren
JBC online July 27th

Baculovirus infection influences host protein expression in two established insect cell lines.
Holly J.R. Popham, James J. Grasela, Cynthia L. Goodman, Arthur H. McIntosh
J. Insect Physiol. (in press)

Wheat germ oil in larval diet influences gene expression in adult oriental fruit fly.
Chiou Ling Chang, Thomas A. Coudron, Cynthia Goodman, David Stanley, Shiheng An, Qisheng Song
Journal of Insect Physiology 56: 356–365

A quantitative mass spectrometry-based approach for identifying protein kinase clients and quantifying kinase activity.*
Yadong Huang, Norma L. Houston, Alejandro Tovar-Mendez, Severin E. Stevenson, Jan A. Miernyk, Douglas D. Randall, Jay J. Thelen
Analytical Biochemistry 402: 69–76

Maize 27 kDa alpha-Zein Is a Potential Allergen for Early Weaned Pigs.
J. Agric. Food Chem. 58: 7323–7328

Regulation of growth response to water stress in the soybean primary root. I. Proteomic analysis reveals region-specific regulation of phenylpropanoid metabolism and control of free iron in the elongation zone
Plant, Cell and Environment 33, 223–243


Confidence assessment for protein identification by using peptide-mass fingerprinting data.
Zhao Song 1, Luonan Chen 2, Dong Xu
Proteomics 9: 3090-99

A rapid method for depletion of Rubisco from soybean (Glycine max) leaf for proteomic analysis of lower abundance proteins.
Krishnan, H.B. and S.S. Natarajan
Phytochemistry 70, 1958–1964

A rapid and simple procedure for the depletion of abundant storage proteins from legume seeds to advance proteome analysis: A case study using Glycine max. **
Krishnan, H.B., N.W. Oehrle and S. Natarajan
Proteomics 9, 3174-3188

Identification of a ß-casein-like peptide in breast nipple aspirate fluid that is associated with breast cancer
Edward R Sauter, Wade Davis, Wenyi Qin, Sarah Scanlon, Brian Mooney, Karen Bromert, and William R Folk
Biomarkers Med. 3 (5) 577-588

Composition, vigor, and proteome of mature soybean seeds developed under high temperature
C. Ren, K. D. Bilyeu and P. R. Beuselinck
Crop Sci 49:1010-1022

System analysis of an Arabidopsis mutant altered in de novo fatty acid synthesis reveals diverse changes in seed composition and metabolism
Mingjie Chen, Brian P. Mooney, Martin Hajduch, Trupti Joshi, Mingyi Zhou, Dong Xu, and Jay J. Thelen
Plant Physiol. 150: 27-41

Peroxiredoxin 2 and Peroxidase Enzymatic Activity of Mammalian Spermatozoa
Gaurishankar Manandhar, Antonio Miranda-Vizuete, Jose R Pedrajas, William J Krause, Shawn Zimmerman, Miriam Sutovsky, Peter Sutovsky
Biol Reprod. 2009 Feb 4 (Epub ahead of print)

Establishment of a Protein Reference Map for Soybean Root Hair Cells
Laurent Brechenmacher, Joohyun Lee, Sherri Sachdev, Zhao Song, Tran Hong Nha Nguyen, Trupti Joshi, Nathan Oehrle, Marc Libault, Brian Mooney, Dong Xu, Bret Cooper, and Gary Stacey
Plant Physiology. 149(2): p. 670-682


Inactivation of N-TIMP-1 by N-terminal acetylation when expressed in bacteria
Steven R. Van Doren, Shuo Wei, Guanghua Gao, Beverly B DaGue, Mark O Palmier, Harinath Badudhanapati, Keith Brew
Biopolymers 89: 960-968

Proteomics of soybean nodule cytosol.
Oehrle, N.W., A.D. Sarma, J.K. Waters and D.W. Emerich.
Phytochemisty 69, 2426-2438

Plant protein isolation and stabilization for enhanced resolution of two-dimensional polyacrylamide gel electrophoresis.
Sarma, A.D., N.W. Oehrle and D.W. Emerich
Analytical Biochemistry 379, 192-195

Maximal Efficiency of Coupling between ATP Hydrolysis and Translocation of Polypeptides Mediated by SecB Requires Two Protomers of SecA.
Mao C, Hardy SJ, Randall LL
J Bacteriol. 2008 Oct 31. [Epub ahead of print]

Anti-chaperone βA3/A1102-117 peptide interacting sites in human αB-crystallin
Guruprasad Rao, Puttur Santhoshkumar, and K. Krishna Sharma
Mol Vis. 2008; 14: 666–674

Significance of Interactions of Low Molecular Weight Crystallin Fragments in Lens Aging and Cataract Formation
Puttur Santhoshkumar, Padmanabha Udupa, Raju Murugesan, and K. Krishna Sharma
J. Biol. Chem. 283: 9477-85

Prostaglandins A1 and E1 influence gene expression in an established insect cell line (BCIRL-HzAM1 cells)
David W. Stanley, Cynthia Goodman, Shiheng An, Arthur McIntosh, and Qisheng Song
Insect Biochemistry and Molecular Biology 38: 275–284


Accumulation of glial fibrillary acidic protein and histone H4 in brain storage bodies of Tibetan terriers with hereditary neuronal ceroid lipofuscinosis
M.L. Katz, D.N. Sanders, B.P. Mooney, G.S. Johnson
J Inherit Metab Dis 30: 952-63

Effect of a single AGE modification on the structure and chaperone activity of human alphaB-Crystallin
Jaya Bhattacharyya, Ekaterina V. Shipova, Puttur Santhoshkumar, K. Krishna Sharma, and Beryl J. Ortwerth
Biochemistry 46: 14682-92

Cell wall proteome in the maize primary root elongation zone. II. Region-specific changes in water soluble and lightly ionically bound proteins under water deficit **
Jinming Zhu, Sophie Alvarez, Ellen L. Marsh, Mary E. LeNoble, In-Jeong Cho, Mayandi Sivaguru, Sixue Chen, Henry T. Nguyen, Yajun Wu, Daniel P. Schachtman, and Robert E. Sharp
Plant Physiology 145: 1533–1548

Ubiquitin C-terminal hydrolase-activity is involved in sperm acrosomal function and anti-polyspermy defense during porcine fertilization
Young-Joo Yi , Gaurishankar Manandhar , Miriam Sutovsky , Rongfeng Li , Vra Jonáková , Richard Oko, Chang-Sik Park, Randall S Prather, and Peter Sutovsky
Biology of Reproduction 77: 780–793

Guest and ligand behavior in Zinc-seamed Pyrogallol[4]arene molecular capsules
Nicholas P. Power, Scott J. Dalgarno, Jerry L. Atwood
Angewandte Chemie International Edition 46: 8601-04

Proteomics of canine lymphoma identifies potential cancer-specific protein markers
Dudley L. McCaw, Arvan S. Chan, Andrew L. Stegner, Brian Mooney, Jeffrey N. Bryan, Susan E. Turnquist, Carolyn J. Henry, Hannah Alexander, and Stephen Alexander
Clin Cancer Res 13: 2496-2503

Proteomic identification of PKC-mediated expression of 20E-induced protein in Drosophila melanogaster
Y Sun, S An, V.C. Henrich, X Sun, Q Song
J Proteome Research 6; 4478-88

Development and assessment of scoring functions for protein identification using PMF data**
Song Z, Chen L, Ganapathy A, Wan XF, Brechenmacher L, Tao N, Emerich D, Stacey G, Xu D.
Electrophoresis 28: 864-70

Kinetics and mechanism of protein Tyrosine Phosphatase 1B inactivation by Acrolein
Derrick R. Seiner, Jason N. LaButti, and Kent S. Gates
Chem Res Toxicol 20: 1315-20


PGAM5, a Bcl-XL-interacting protein, is a novel substrate for the redox-regulated Keap1-dependent ubiquitin ligase complex
Shih-Ching Lo and Mark Hannink
J.Biol. Chem. 281: 37893-903

A comparative proteomic evaluation of culture grown vs nodule isolated Bradyrhizobium japonicum
Annamraju Sarma and David Emerich
Proteomics 6: 3008-28

Sites of interaction of a precursor polypeptide on the export chaperone SecB mapped by site-directed spin labeling
Jennine M. Crane, Yuying Suo, Angela A. Lilly, Chunfeng Mao, Wayne L. Hubbell, and Linda L. Randall
J. Mol. Biol. 363: 63–74

Characterization of three areas of interactions stabilizing complexes between SecA and SecB, two proteins involved in protein export
Chetan N. Patel, Virginia F. Smith and Linda L. Randall
Protein Sci. 15: 1379-1386

Using quantitative proteomics of Arabidopsis roots and leaves to predict metabolic activity
Mooney B.P., Miernyk J.A., Greenlief C.M., Thelen, J.J.
Physiologia Plantarum 128: 237–250

Chemical cross-linking immobilized concanavalin A for use in proteomic analyses
Jan A Miernyk, Mark L Johnston
Preparative Biochem and Biotechnol 36: 2003-14

Characterization of recombinant Francisella tularensis acid phosphatase A
Thomas J. Reilly, Richard L. Felts, Michael T. Henzl, Michael J. Calcutt, and John J. Tanner
Protein Expression and Purification Volume 45: 132-141


Proteomic analysis of soybean root hairs after infection by Bradyrhizobium japonicum
J Wan, M Torres, A Ganapathy, J Thelen, BB DaGue, B Mooney, D Xu, and G Stacey
Mol Plant Microbe Interact 18: 458-67.

Mapping of the docking of SecA onto the chaperone SecB by site-directed spin labeling: Insight into the mechanism of ligand transfer during protein export
Jennine M. Crane, Chunfeng Mao, Angela A. Lilly, Virginia F. Smith, Yuying Suo, Wayne L. Hubbell, and Linda L. Randall
J Mol Biol 2: 295-307

Expression and proteasomal degradation of the major vault protein (MVP) in mammalian oocytes and zygotes
Peter Sutovsky, Gaurishankar Manandhar, Jozef Laurincik, Juraj Letko, Jose Nestor Caamaño, Billy N Day, Liangxue Lai, Randall S Prather, Kathy L Sharpe-Timms, Randall Zimmer, and Miriam Sutovsky
Reproduction 129: 269-282

Golgi-to-late endosome trafficking of the yeast pheromone processing enzyme Ste13p is regulated by a phosphorylation site in its cytosolic domain
Holly D. Johnston, Christopher Foote, Andrea Santeford and Steven F. Nothwehr
Molecular Biology of the Cell 16: 1456–1468

Global protein expression pattern of Bradyrhizobium japonicum bacteroids: A prelude to functional proteomics
Annamraju D. Sarma and David W. Emerich
Proteomics 5: 4170-84


High-throughput peptide mass fingerprinting of soybean seed proteins: automated workflow and utility of UniGene expressed sequence tag databases for protein identification
Mooney B P, Krishnan H, and Thelen J J
Phytochemistry 65: 1733-44

Proteomic analysis to identify breast cancer biomarkers in Nipple Aspirate Fluid
Hannah Alexander, Andrew L. Stegner, Colette Wagner-Mann, Garrett C. Du Bois, Stephen Alexander, and Edward R. Sauter
Clinical Cancer Research 10: 7500-10

Statistical assessment for mass-spec protein identification using peptide fingerprinting approach
Ganapathy A, Wan XF, Wan J, Thelen J, Emerich DW, Stacey G, Xu D.
Conf Proc IEEE Eng Med Biol Soc. 4: 3051-4


**Staff consultation was the only contribution to this manuscript