photo of ThermoFisher Scientific LTQ Orbitrap XL 

ThermoFisher Scientific LTQ Orbitrap XL

  • Hybrid ion trap, Orbitrap mass spectrometer
  • Accurate mass instrument (routinely < 5ppm on precursor)
  • High resolution, typically 30,000 for peptides
  • Very high resolution possible (>100,000 at m/z 400)
  • Reversed-phase separation of peptides using integrated Proxeon nano-HPLC
photo of Agilent 6520 Accurate Mass QTOF 

Agilent 6520 Accurate Mass QTOF

  • Hybrid quadrupole time-of-flight mass spectrometer
  • Integrated nano-HPLC
  • Integrated CHIP CUBE (microfluidic C18 column for peptide separation)
  • typical peptide resolution >10,000
  • typical peptide mass accuracy <5 ppm

photo of Amersham IPGphor & Bio-Rad Protean IEF


Amersham IPGphor & Bio-Rad Protean IEF

  • Isoelectric focusing units
photo of Amersham SE 600, Ettan DALTsix, & SE62 

Amersham SE 600, Ettan DALTsix, & SE62

  • Second-dimension gel electrophoresis and electrophoretic transfer units
photo of Fujifilm FLA5000 

Fujifilm FLA5000

  • Laser imaging system (three lasers, dual photomultiplier tubes)
  • Capable of imaging Cy2, Cy3, Cy5 dyes, Sypro, ProQ, etc. fluorescent stains
  • Phosphoimaging capability (NO radioactive samples are allowed in the Center)
photo of ABI Voyager-DE Pro 

ABI Voyager-DE Pro

  • Matrix-assisted laser desorption ionization time-of-flight mass spectrometer
  • Linear and reflector modes
  • Capable of high-throughput peptide mass fingerprinting
  • Capable of intact mass determination of proteins
photo of Applied Biosystems 4700 MALDI TOF-TOF 

Applied Biosystems 4700 MALDI TOF-TOF

  • MALDI tandem time-of-flight mass spectrometer
  • Linear and reflector modes
  • MS and MS/MS modes
  • High-throughput instrument (192 samples in MS mode in about 2 hours)
photo of Thermo Quantiva 

Thermo Quantiva

  • Triple quadrupole mass spectrometer
  • Nano-HPLC with ESI source
  • Targeted quantitation of peptides/proteins
  • Aqua-MRM. Used for relative quantitation between samples, can also be used for absolute quantitation (i.e. µ/mL etc.) by using 13C-labeled peptides as internal standards allowing very accurate quantitation of proteins
photo of Waters Xevo TQ-S 

Waters Xevo TQ-S

  • Triple quadrupole mass spectrometer
  • UHPLC with ESI or APCI source
  • High sensitivity and quantitation of targeted compounds in both positive and negative ion modes
  • Part of the Small Molecule MS Lab