ThermoFisher Scientific LTQ Orbitrap XL


• Hybrid ion trap, Orbitrap mass spectrometer
• Accurate mass instrument (routinely < 5ppm on precursor)
• High resolution, typically 30,000 for peptides
• Very high resolution possible (>100,000 at m/z 400)
• Reversed-phase separation of peptides using integrated nano-HPLC (See Dionex Ultimate 3000 below)

Agilent 6520 Accurate Mass QTOF


• Hybrid quadrupole time-of-flight mass spectrometer
• Integrated nano-HPLC
• Integrated CHIP CUBE (microfluidic C18 column for peptide separation)
• typical peptide resolution >10,000
• typical peptide mass accuracy <5 ppm

Amersham IPGphor & Bio-Rad Protean IEF


• Isoelectric focusing units

Amersham SE 600, Ettan DALTsix, & SE62


• Second-dimension gel electrophoresis and electrophoretic transfer units.

Genetix GelPix


• Spot-cutting robot
• Captures both visible and fluorescent images in 16-bit TIF format
• Phoretix (Nonlinear Dynamics) image analysis software
• Throughput of 400 spots per hour
  

Fujifilm FLA5000

• Laser imaging system (three lasers, dual photomultiplier tubes)
• Capable of imaging Cy2, Cy3, Cy5 dyes, Sypro, ProQ, etc. fluorescent stains
• Phosphoimaging capability (NO radioactive samples are allowed in the Center)
  

   

Dionex UltiMate 3000

• Nano and capillary LC separation system
• Capable of ion-exchange and reversed-phase separations
• Integrated with LTQ Orbitrap
  
  

ABI Voyager-DE Pro


• Matrix-assisted laser desorption ionization time-of-flight mass spectrometer
• Linear and reflector modes
• Capable of high-throughput peptide mass fingerprinting
• Capable of intact mass determination of proteins
  
  

Applied Biosystems 4700 MALDI TOF-TOF

• MALDI tandem time-of-flight mass spectrometer
• Linear and reflector modes
• MS and MS/MS modes
• High-throughput instrument (192 samples in MS mode in about 2 hours)
  
  

ABI QStar


• Electrospray ionization quadrupole time-of-flight tandem mass spectrometer
• Capillary LC, nanospray ESI sources